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光谱检测分析仪
HORIBA荧光光谱仪QuantMaster 8000
Orient KOJI液氮低温杜瓦附件LN2030
HORIBA三维荧光及吸收光谱仪Duetta
飞秒瞬态吸收/拉曼光谱仪fs-TAS
高效型CL光谱仪HCLUE
超快成像拉曼光谱仪-XploRA PLUS XS
射频辉光放电光谱仪GD-Profiler HR
模块式荧光光谱仪-Fluorolog-3
HORIBA JY高性能荧光寿命分析仪-DeltaPro
HORIBA科研级荧光光谱仪FluoroMax+
光学仪器及设备
ISS三维纳米成像及单分子跟踪套件
ISS升级激光共焦扫描显微镜到FLIM/FCS系统
ISS超分辨激光共焦扫描显微镜Alba-STED
滨松C12741-03 InGaAs红外相机
ISS激光共聚焦快速荧光寿命成像系统FLIM/FCS
滨松sCMOS相机ORCA-Flash4.0 LT
滨松sCMOS相机ORCA-Flash 4.0 V2
显微拉曼荧光成像综合测试系统
光学平台
上转换荧光寿命测试模块
元素分析仪
SLFA-UV21A紫外荧光法硫分析仪
EMIA-220V红外碳硫分析仪
EMIA-320V红外碳硫分析仪
EMIA-820V红外碳硫分析仪
EMGA-600W氧/氮/氢联合分析仪
EMGA-620W/C氧/氮分析仪
EMGA-620W氧/氮分析仪
激光粒度仪
大塚小角激光散射仪PP-1000
Horiba激光散射粒度分布分析仪(LA-950V2)
HORIBA LA960激光粒度仪
HORIBA纳米粒度分析仪
HORIBA激光散射粒度分布分析仪
X射线仪器
HORIBA X射线分析显微镜
HORIBA X-ray荧光光谱仪
制冷设备
Oxford无液氦光谱学恒温器Optistat Dry
Cryo-77液氮低温恒温器
石油专用分析仪器
SLFA-20荧光X射线硫分析仪
SLFA-2100/2800荧光X射线硫分析仪
成像系统
时间分辨小动物活体荧光成像系统i-NIR-II
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单分子检测水平的定量细胞生物学检测仪器。
受激发射损耗(STED)是一种强大的显微技术,可以观察到空间分辨率低于衍射极限的荧光结构。Alba-STED使用脉冲激发和脉冲耗尽方法(pSTED)结合数字频域荧光寿命成像(FastFLIM)来记录时间分辨光子,从而提高图像分辨率并分离具有相同激发波长的两个荧光标记物。
关键特色 : Alba-STED for FLIM/FFS:
• pSTED (Pulsed excitation and pulsed STED)
• FastFLIM for time-resolved pSTED acquisition
• Improved image resolution using the phasor plot
• Dual-label excitation
• Fast image acquisition (dwell time: 0.2 μs)
• High dynamic range (signal up to 60 million counts/s)
测试实例展示 Alba-STED for FLIM/FFS
Confocal (left) vs. pSTED (right) images of the actin labeled with the SiR dye in fixed glia cells, acquired by FastFLIM.
Confocal (left) vs. pSTED (right) images of the actin labeled with the SiR dye in fixed glia cells, acquired by FastFLIM.
Confocal images of 60nm fluorescence beads (left); pSTED images (middle); sharpening the pSTED image using a binary filter based on the phasor plots (right).
Dual labels can be separated using pSTED and FastFLIM. Atto 647N and Atto 655 were used as labels; they both are excited by the 640 nm laser. The two dyes are first separated using the phasor plots, and then assigned with two different false colors (Atto 647N - yellow, Atto 655 - purple) to produce the processed and merged pSTED image of the two labels.
参考论文:
Monomeric cohesin state revealed by live-cell single-molecule spectroscopy.
Liu, W., Biton, E., Pathania, A., Matityahu, A., Irudayaraj, J., Onn, I.
EMBO Rep. 2019 Dec 29:e48211. doi: 10.15252/embr.201948211. [Epub ahead of print]
Photon-separation to enhance the spatial resolution of pulsed STED microscopy.
Tortarolo, G., Sun, Y., Teng, K.W., Ishitsuka, Y., Lanzanó, L., Selvin, P.R., Barbieri, B., Diaspro, A., Vicidomini, G.
Nanoscale. 2019 Jan 9. doi: 10.1039/c8nr07485b. [Epub ahead of print]
A straightforward STED-background corrected fitting model for unbiased STED-FCS analyses.
Wang, R., Brustlein, S., Mailfert, S., Fabre, R., Fallet, M., Sivankutty, S., Rigneault, H., Marguet, D.
Methods. 2018 May 1;140-141:212-222. doi: 10.1016/j.ymeth.2018.02.010. Epub 2018 Feb 14.
A Novel Pulsed STED Microscopy Method Using FastFLIM and the Phasor Plots
Sun, Y.,Tortarolo G., Teng, K.-W., Ishitsuka, Y., Coskun, U.C., Liao, S.-C., Diaspro, A., Vicidomini, G., Selvin, P.R., Barbieri, B.
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100691C (February 21, 2017)